scholarly journals Prognostic significance of DNA ploidy and proliferation in 309 colorectal carcinomas as determined by two-color multiparametric DNA flow cytometry

Cancer ◽  
1997 ◽  
Vol 79 (11) ◽  
pp. 2073-2086 ◽  
Author(s):  
Richard J. Zarbo ◽  
Raouf E. Nakhleh ◽  
Ronald D. Brown, James J. Kubus, Ch Ma ◽  
Patricia Mackowiak
Keyword(s):  
Flow Cytometry ◽  
Dna Ploidy ◽  
Prognostic Significance ◽  
Dna Flow Cytometry ◽  
Colorectal Carcinomas

Two-Color, Cytokeratin-Labeled DNA Flow Cytometric Analysis of 332 Breast Cancers

2001 ◽  
Vol 125 (3) ◽  
pp. 364-374
Author(s):  
Anil R. Prasad ◽  
George Divine ◽  
Richard J. Zarbo
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Tumor Size ◽  
Dna Ploidy ◽  
Prognostic Significance ◽  
Consensus Conference ◽  
Histologic Grade ◽  
Dna Flow Cytometry ◽  
Axillary Lymph ◽  
T Classification

Abstract Context.—DNA flow cytometry of breast cancer is a proposed tumor marker of prognostic significance that is of controversial clinical utility because of lack of standardization and confirmatory studies. Objective.—To evaluate the prognostic significance of the more informative technique of multiparametric 2-color DNA flow cytometry as recommended by the 1992 DNA Cytometry Consensus Conference. Design.—Three hundred thirty-two breast carcinomas with 7 to 12 years of follow-up were prospectively analyzed as fresh tumors that were mechanically dissociated into whole cell suspensions. These suspensions were dual fluorescence–labeled with propidium iodide (DNA) and antibodies to cytokeratin (epithelium) and leukocyte common antigen (internal leukocyte control) for gated analysis of subpopulations. Multicycle software with histogram-dependent algorithms employing background, aggregate, and debris correction were used in DNA and cell-cycle quantitation. Data were analyzed according to the DNA Flow Cytometry Consensus Conference recommendations. Results.—DNA ploidy and proliferation stratified into 3 categories were not predictive of overall or disease-free survival. Sixty-five percent of tumors were nondiploid, and 35.4% were diploid. Two hundred six tumors were able to be evaluated for synthesis-phase fraction (SPF) analysis, with 74 of 206 cases in the low range (<13.4%), 36.4% in the intermediate range (>13.5 to <25.4%), and 27.6% in the high SPF (>25.5%) category. Aneuploid tumors tended to have a higher SPF. Univariate survival analysis showed prognostic significance of the following: tumor size, stage, TNM components, vascular invasion, nuclear grade, and histologic grade. Only T classification, presence of positive axillary lymph nodes, and distant metastases were significant independent predictors of survival in multivariate Cox regression models. Age and hormone receptor status showed no prognostic significance. Synthesis-phase fraction was significantly correlated with tumor size, stage, T classification, nuclear and histologic grade, presence of estrogen or progesterone receptors, and axillary lymph node status. None of the histologic parameters showed any significant association with DNA aneuploidy, except for high nuclear and histologic grade and the absence of estrogen receptors. Conclusions.—Despite the use of state-of-the-art processing and flow cytometry analytic techniques, DNA ploidy and proliferation measurements were not predictive of survival in any stage of breast cancer. However, select histopathologic parameters and TNM stage were significant predictors of survival in univariate and multivariate analyses. We conclude that DNA ploidy and proliferation measurements do not provide significant prognostic information for clinicians to integrate into therapeutic decision making for patients with breast cancer.

Flow cytometric DNA analysis in conservatively treated renal tumours

1995 ◽  
Vol 62 (1_suppl) ◽  
pp. 141-143
Author(s):  
P. Beltrami ◽  
M. Lazzarotto ◽  
G. Giusti ◽  
C. Tallarigo ◽  
G. Malossini ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Analysis ◽  
Tumour Size ◽  
Prognostic Significance ◽  
Dna Flow Cytometry ◽  
Additional Parameter ◽  
Flow Cytometric ◽  
Renal Tumours ◽  
Dna Histograms

— The DNA histograms of 21 conservatively resected renal tumours were studied using DNA flow cytometry. Five patients had an imperative and sixteen an elective indication for conservative resection of the renal tumour. On the basis of DNA histograms twelve aneuploid tumours were pointed out. A mean follow-up of 34.2 months was considered to see whether the ploidy would provide criteria with a prognostic significance, to be useful as an additional parameter. None of the twenty-one patients had local recurrence or distant metastasis: in our series the DNA analysis had no influence on the prognosis of this group of patients. The tumour size seems to be the only selective parameter for choosing renal-conserving surgery.

Prognostic value of DNA flow cytometry in the locally recurrent, conservatively treated breast cancer patient.

1992 ◽  
Vol 10 (12) ◽  
pp. 1839-1847 ◽  
Author(s):  
B G Haffty ◽  
M Toth ◽  
S Flynn ◽  
D Fischer ◽  
D Carter
Keyword(s):  
Breast Cancer ◽  
Flow Cytometry ◽  
Survival Rate ◽  
Local Recurrence ◽  
Dna Ploidy ◽  
Disease Free Survival ◽  
Dna Flow Cytometry ◽  
Free Survival ◽  
Locally Recurrent ◽  
Disease Free

PURPOSE This study attempted to determine the prognostic value of DNA flow cytometry in the treatment of patients with locally recurrent, conservatively treated breast cancer. METHODS AND MATERIALS Of 433 patients with clinical stage I and II breast cancer treated with conservative surgery and radiotherapy at Yale-New Haven Hospital before January 1985, 50 patients experienced an ipsilateral breast relapse as a first site of treatment failure. Using standard flow-cytometric techniques, DNA ploidy, DNA index, and S-phase fraction (SPF) were measured for 38 of the 50 (76%) paraffin-embedded specimens available for analysis. RESULTS At a median postrecurrence follow-up of 5.8 years, the 5-year and disease-free survival rates following ipsilateral breast treatment failure were 48% and 54%, respectively. Sixty-three percent of the recurrent tumors were DNA diploid and 37% were aneuploid. Both DNA ploidy and SPF were statistically significant prognostic indicators for 5-year survival and disease-free survival after local recurrence. The 5-year survival rate of the DNA diploid population was 64%, compared with 15% in the aneuploid population (P < .02). Patients with low SPF (< 12%) experienced an 83% 5-year survival rate, compared with a 24% 5-year survival rate in patients with high SPF (> or = 12%) (P < .03). Ploidy and SPF were combined to define the categories of favorable (diploid, low SPF) and unfavorable (diploid, high SPF or any aneuploid subgroups). Patients in the favorable category experienced an 89% 5-year postrecurrence survival rate and a 100% disease-free survival rate, whereas patients in the unfavorable category had a 24% 5-year survival rate and a 32% disease-free survival rate (P < .01). The flow cytometry as a factor correlated with other clinical parameters previously shown to be of prognostic significance in this patient population. In a multivariate analysis, flow cytometry was a statistically significant and independent prognostic factor for disease-free survival following local recurrence. CONCLUSIONS DNA ploidy and SPF as measured by currently available flow-cytometric techniques show promise as a tool in determining prognosis for the patient with locally recurrent breast cancer. Implications of these findings with respect to issues of adjuvant systemic therapy at the time of local recurrence are discussed.

L'analisi del DNA mediante citometria a flusso nei tumori superficiali della vescica: DNA analysis through flow cytometry in superficial bladder tumours

1995 ◽  
Vol 62 (2) ◽  
pp. 188-195
Author(s):  
R. Pianon ◽  
A. D'Amico ◽  
M. Rahmati ◽  
A. Paganelli ◽  
D. Schiavone ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Clinical Practice ◽  
Dna Analysis ◽  
Prognostic Significance ◽  
Clinical Development ◽  
Dna Flow Cytometry ◽  
Through Flow ◽  
Data Investigation ◽  
Precise Role ◽  
Bladder Tumours

The aim of this work is to try to characterize the prognostic value of DNA flow cytometry in superficial bladder tumours and to try to indicate this investigation in clinical practice. The Authors review results reported in literature and those relative to their experience. Data investigation shows that this test has prognostic significance because it is correlated with the stage and grade of the tumour. The correlation with the clinical development of the tumour is not clear in the Authors’ experience. The Authors think that flow cytometry does not have a precise role in clinical practice.

dna flow cytometry of canine mammary tumours: the relationship of dna ploidy and S-phase fraction to clinical and histological features

1995 ◽  
Vol 58 (3) ◽  
pp. 238-243 ◽  
Author(s):  
M.D Péreze Alenza ◽  
G.R Rutteman ◽  
N.J Kuipers-Dijkshoorn ◽  
L Peña ◽  
A Montoya ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Dna Flow Cytometry ◽  
Histological Features ◽  
Mammary Tumours ◽  
Relationship Of ◽  
The Relationship

scholarly journals Correlation of DNA Ploidy with Progression of Cervical Cancer

2008 ◽  
Vol 2008 ◽  
pp. 1-7 ◽  
Author(s):  
M. Singh ◽  
S. Mehrotra ◽  
N. Kalra ◽  
U. Singh ◽  
Y. Shukla
Keyword(s):  
Cervical Cancer ◽  
Flow Cytometry ◽  
Squamous Cell ◽  
Dna Ploidy ◽  
Flow Cytometric Analysis ◽  
Biological Behavior ◽  
Dna Flow Cytometry ◽  
High Risk Population ◽  
Severe Dysplasia ◽  
Cervical Smear

The majority of squamous cell carcinomas of cervix are preceded by visible changes in the cervix, most often detected by cervical smear. As cervical cancer is preceded by long precancerous stages, identification of the high-risk population through detection of DNA ploidy may be of importance in effective management of this disease. Here we attempted to correlate aneuploid DNA patterns and their influence on biological behavior of flow-cytometry analysis of DNA ploidy which was carried out in cytologically diagnosed cases of mild (79), moderate (36), and severe (12) dysplasia, as well as “atypical squamous cells of unknown significance (ASCUS)” (57) along with controls (69), in order to understand its importance in malignant progression of disease. Cytologically diagnosed dysplasias, which were employed for DNA ploidy studies, 39 mild, 28 moderate, and 11 severe dysplasia cases were found to be aneuploid. Out of the 69 control subjects, 6 cases showed aneuploidy pattern and the rest 63 subjects were diploid. An aneuploidy pattern was observed in 8 out of 57 cases of cytologically evaluated ASCUS. The results of the followup studies showed that aberrant DNA content reliably predicts the occurrence of squamous cell carcinoma in cervical smear. Flow cytometric analysis of DNA ploidy may provide a strategic diagnostic tool for early detection of carcinoma cervix. Therefore, it is a concept of an HPV screening with reflex cytology in combination with DNA flow cytometry to detect progressive lesions with the greatest possible sensitivity and specificity.

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